GS_00G4PLE.2

USA/GS_00G4PLE.2/2025-09-07
A. P. Rothstein, S. Collins, G. Muir, A. Simas, B. Lu, B. Cosca, B. Girinathan, P. Leeson, A. M. Plocik & B. Simen

Ginkgo Bioworks, Ginkgo Biosecurity

Submission details

Submission ID
PX422963.1.seg1/PX422962.1.seg2/PX422961.1.seg3/PX422956.1.seg4/PX422959.1.seg5/PX422958.1.seg6/PX422957.1.seg7/PX422960.1.seg8
Submitting group
Date submitted
2025-11-27 16:45:45 UTC
Date released
2025-11-27 16:51:55 UTC

Clade

Clade HA
D.3.1
Clade NA
D.2

Sample details

Collection country
USA
Collection subdivision level 1
Florida
Collection date
2025-09-07
Isolate name
A/Florida/GKISBBBH04250/2025

Host

Host

INSDC

INSDC accession seg1
INSDC accession seg2
INSDC accession seg3
INSDC accession seg4
INSDC accession seg5
INSDC accession seg6
INSDC accession seg7
INSDC accession seg8
NCBI release date
2025-09-26

INSDC seg4

subtype_seg4
H1

INSDC seg6

subtype_seg6
N1

Alignment and QC

Length seg1
2318 (100%)
Total inserted nucs seg1
38
Total SNPs seg1
86
Length seg2
2316 (100%)
Total inserted nucs seg2
42
Total SNPs seg2
67
Length seg3
2208 (100%)
Total inserted nucs seg3
57
Total SNPs seg3
80
Length seg4
1764 (100%)
Total inserted nucs seg4
12
Total SNPs seg4
113
Length seg5
1542 (100%)
Total inserted nucs seg5
45
Total SNPs seg5
59
Length seg6
1433 (100%)
Total SNPs seg6
22
Length seg7
1002 (100%)
Total inserted nucs seg7
20
Total SNPs seg7
26
Length seg8
878 (100%)
Total inserted nucs seg8
15
Total SNPs seg8
37

Nucleotide mutations

Mutations called relative to the NC_026438.1, NC_026435.1, NC_026437.1, CY121680.1, NC_026436.1, MW626056.1, NC_026431.1 & NC_026432.1 references

Substitutions

seg1

  • A18G
  • A90C
  • A96G
  • A108G
  • G123A
  • A132G
  • A138G
  • G161A
  • A174G
  • A194G
  • G198A
  • T201C
  • A225G
  • C242T
  • A243G
  • C285A
  • A291G
  • C309T

seg2

  • T12C
  • G42A
  • A159G
  • G168A
  • G180A
  • C192T
  • T207A
  • T210C
  • T222C
  • G225A
  • C297T
  • G312A
  • A345G
  • A366G
  • T384C
  • T397C
  • C447T
  • T450C

seg3

  • G40A
  • R53A
  • T81C
  • G84C
  • A88G
  • C90T
  • C99T
  • A114T
  • G126A
  • C153T
  • C162T
  • C165T
  • G187A
  • G204A
  • T285C
  • G298A
  • G423A
  • G477A

seg4

  • G39A
  • C42A
  • A47G
  • G57A
  • A119G
  • T161C
  • C164T
  • G188A
  • A231C
  • C293G
  • C318T
  • G322A
  • T353C
  • G360A
  • A408C
  • G416A
  • A429G
  • C443T

seg5

  • A33G
  • G42T
  • C87T
  • A97G
  • C141A
  • C171T
  • T177C
  • C180T
  • C249A
  • A288C
  • G298A
  • G309A
  • T314C
  • G315A
  • R341A
  • T365A
  • A390G
  • T402C

seg6

  • T50C
  • A144G
  • G163A
  • G264A
  • A445G
  • C452A
  • G607A
  • A785G
  • T799C
  • C809T
  • A818G
  • C893T
  • G914T
  • G969A
  • T1016C
  • A1049G
  • T1163C
  • C1187T

seg7

  • G75A
  • C124A
  • G238A
  • G258A
  • A293G
  • G306A
  • G339A
  • C375A
  • T432C
  • G441T
  • G492A
  • C507A
  • A574G
  • G600A
  • C622A
  • G660A
  • T663C
  • G687A

seg8

  • C6A
  • C42T
  • C54T
  • C57T
  • T99C
  • C108A
  • T150A
  • C153T
  • T159C
  • G163A
  • C192T
  • C204T
  • A238G
  • G250A
  • C264T
  • C268A
  • C315T
  • G363A
Deletions
N/A
Insertions

seg1

ins_0:GGTCAAATATATTCAAT, ins_2280:TGTCGAATTGTTTAAAAACGA

seg2

ins_0:CAAACCATTTGA, ins_2274:TGAATTTAACTTGTCCTTCATGAAAAAATG

seg3

ins_0:TACTGATCCAAA, ins_2151:TTGTGGCAATGCTACTATTTGCTATCCATATTGTCCAAAAAAGTA

seg4

ins_0:AGCGAAAGCAGG

seg5

ins_0:GGGTAGATAATCACTCAATGAGTGACATCGAAGCC, ins_1497:AGAAAAATAC

seg7

ins_0:TAGATGTTTAAAG, ins_982:AAAACTA

seg8

ins_0:GTGACAAAAACATA, ins_863:T

Amino acid substitutions

Substitutions called relative to the NC_026438.1, NC_026435.1, NC_026437.1, CY121680.1, NC_026436.1, MW626056.1, NC_026431.1 & NC_026432.1 references

Substitutions

HA1

  • HA1:K54Q
  • HA1:S74R
  • HA1:P83S
  • HA1:S84N
  • HA1:D97N
  • HA1:T120A
  • HA1:N129D
  • HA1:K130N
  • HA1:P137S
  • HA1:K142R
  • HA1:N156K
  • HA1:L161I
  • HA1:S162N
  • HA1:K163Q
  • HA1:S164T
  • HA1:S183P
  • HA1:S185I
  • HA1:A186T

HA2

  • HA2:E29D
  • HA2:I45V
  • HA2:E47K
  • HA2:I91V
  • HA2:S124H
  • HA2:I133T
  • HA2:N169S
  • HA2:E172K
  • HA2:E179D
  • HA2:V193A

M1

  • M1:L42I
  • M1:V80I
  • M1:K98R
  • M1:M192V
  • M1:Q208K
  • M1:K230R

M2

  • M2:D21G
  • M2:S23N

NA

  • NA:I46V
  • NA:S52N
  • NA:A86T
  • NA:N146S
  • NA:S200N
  • NA:I264T
  • NA:V321I
  • NA:V453M
  • NA:K469N

NEP

  • NEP:D2E
  • NEP:N29S
  • NEP:T48A
  • NEP:M83I

NP

  • NP:E14D
  • NP:I33V
  • NP:V100I
  • NP:M105T
  • NP:X114E
  • NP:L122Q
  • NP:I136L
  • NP:V217I
  • NP:N377S
  • NP:V425I
  • NP:S498N

NS1

  • NS1:D2E
  • NS1:E55K
  • NS1:T80A
  • NS1:V84I
  • NS1:L90I
  • NS1:I123V
  • NS1:E125D
  • NS1:K131E
  • NS1:A155T
  • NS1:N205S

PA

  • PA:V14I
  • PA:X18E
  • PA:I30V
  • PA:V63I
  • PA:V100I
  • PA:P224S
  • PA:S225C
  • PA:R262K
  • PA:L268F
  • PA:N321K
  • PA:I330V
  • PA:A337T
  • PA:K339N
  • PA:R362K
  • PA:I438V
  • PA:I505V

PA-X

  • PA-X:V14I
  • PA-X:X18E
  • PA-X:I30V
  • PA-X:V63I
  • PA-X:V100I
  • PA-X:R199K
  • PA-X:N204S
  • PA-X:R206K
  • PA-X:R221Q
  • PA-X:L229S

PB1

  • PB1:G154D
  • PB1:V200I
  • PB1:K386R
  • PB1:I397M
  • PB1:I435T
  • PB1:K757R

PB2

  • PB2:R54K
  • PB2:D65G
  • PB2:M66I
  • PB2:T81M
  • PB2:D195N
  • PB2:G225S
  • PB2:R293K
  • PB2:R299K
  • PB2:V344M
  • PB2:I354L
  • PB2:S453T
  • PB2:L475M
  • PB2:V667I
  • PB2:V731I

SigPep

  • SigPep:V7I
  • SigPep:L8M
  • SigPep:A13T
Deletions
N/A
Insertions
N/A